Composite

Part:BBa_J36847

Designed by: Perry Tsai   Group: iGEM06_Harvard   (2006-10-30)

Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6

Assembled construct of signal peptide of lipoprotein, five transmembranes domain of outer membrane protein A, and streptavidin, single-chain dimeric with His6 tag at the end.

This construct codes for a protein which displays streptavidin on the cell surface.

NOTE ABOUT THE SEQUENCE: The mixed site between parts is 'only' six base pairs, ACTAGA. There is no spacer T or G nucleotide. These spacer nucleotides have been placed in the results for "get selected sequence" as an automatic composite-parts addition for the BioBricks mixed site between assembled parts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1241
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 662
    Illegal AgeI site found at 1196
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None